经腹入路两种途径行腹腔镜下左侧输尿管切开取石术在学习初期的比较

目的对经验不足的外科医生,比较腹腔镜下经腹入路肠系膜间途径行输尿管切开取石术与传统经腹入路侧结肠途径行输尿管切开取石术的疗效。方法回顾性分析郑州大学附属洛阳中心医院在2014年3月至2018年3月期间收治的59例左侧肾盂输尿管连接部狭窄患者的资料,采用常规侧结肠入路32例,而经中结肠系膜间入路27例,比较围手术期结果和随访资料,比较两种方法患者的手术时间、术中失血量、术后肠道功能恢复,术后疼痛程度等情况。结果经中结肠入路组手术时间明显短于常规侧结肠入路组(P<0.05),经中结肠系膜间入路组术后疼痛明显减轻,两组患者在术中失血量、术后进食时间、住院时间等方面差异均无统计学意义(P>0.05)。结论通过中结肠系膜间途径直接暴露输尿管可节省游离侧结肠所需时间。该方法对于经验有限的外科医生也是安全可行的,并具有与侧结肠手术相似的成功率。     

Genomic analysis of a recombinant NADC30-like porcine reproductive and respiratory syndrome virus in china

Recently, NADC30-like porcine reproductive and respiratory syndrome viruses (PRRSVs), which are genetically similar to the NADC30 strain isolated in the United States of America in 2008, have become prevalent in China. Here, a novel variant PRRSV strain named HNhx was successfully isolated on porcine alveolar macrophages from Henan province and the full-length genome sequence was determined. Phylogenetic analysis indicated that HNhx strain was classified into the NADC30-like PRRSV subgroup, in which all the strains had the unique discontinuous 131-amino acid deletion relative to that of the nonstructural protein 2 (Nsp2) of the VR2332 strain. Genetically, HNhx shared 92.9% nucleotide similarity to NADC30. Furthermore, HNhx strain contained extensive amino acid mutations in GP5. In particular, the S32H, N33D, D34N, and S36G variations resulted in that HNhx lost all the putative N-linked glycosylation sites at amino acid positions 30, 32, 33, 34, and 35. Recombination analysis revealed that HNhx was the result of recombination between the NADC30 strain and the highly pathogenic PRRSV vaccine strain circulating in China in Nsp4 (nt 5261) to Nsp9 (nt 7911). The novel genome data of HNhx will be helpful for understanding the evolution and epidemiology of PRRSV in China.     

Screening HCV genotype-specific epitope peptides based on conserved sequence analysis and B cell epitope prediction in HCV E2 region

The high mutation rate of the hepatitis C virus (HCV) genome increases the genotype diversity and renders the detection of the virus more difficult. Therefore, prediction and assessment of highly conserved and strongly antigenic epitope polypeptide sequences have become a focus of current research. The E2 region is the target binding region of neutralizing antibodies. HCV genomics, especially the high mutation rate of E2 region sequence, makes its genotyping more and more diverse, and the detection of HCV and genotype is becoming more and more strict. In this study, four HCV B cell epitope polypeptides were constructed based on assessment of conserved sequences in the HCV E2 region and prediction of B cell epitopes, including sequences specific to genotype 1A (DC-13: 434-DTGWLAGLFYYHK-446), genotype 1B (HC-13: 434-HTGFLAALFYAKS-446), genotype 4D (NC-13: 434-NTGFLASLFYTHK-446), and a consensus sequence (FC-9: 447-FNSSGCPER-455). Epitope polypeptides combined with serum from 29 HCV-infected or 25 non-HCV-infected individuals were assayed by enzyme-linked immunosorbent assay (ELISA), and differences were analyzed by T/T’ test methods in SPSS v20.0 software. Binding levels of genotype 1A, 4D, and consensus epitope polypeptides with sera of HCV-infected patients were higher than those of non-infected individuals. Moreover, binding of genotype 1B epitope polypeptides with serum of HCV 1B-infected patients was higher than that of HCV 2A-infected patients. While the screening results of HCV genotype-specific epitope polypeptides were preliminary, these findings indicated that we successfully established an HCV and genotype serological ELISA detection method. Such an approach would facilitate the discovery of epitope polypeptides which may become new antigen candidates in peptide vaccine development for the prevention of HCV infection.     

脂氧素及其在牙周炎症消退中的作用

脂氧素（LX）是调节炎症消退的重要内源性脂质抗炎递质,被称作炎症的“刹车”信号。局部应用LX类似物后,牙槽骨破坏明显减轻,且炎症细胞和免疫细胞对牙周结缔组织的浸润也明显减少,即LX在促进牙周炎症的消退中起着不可忽视的作用。     

Ophiopogonin D Inhibiting Epithelial NF-κB Signaling Pathway Protects Against Experimental Colitis in Mice

Inflammation - The sustained activation of the nuclear factor κB (NF-κB) signaling pathway has been observed in human inflammatory bowel disease (IBD). Ophiopogonin D (OP-D) is a small...     

Development of Laboratory Parameters-Based Formulas in Predicting Short Outcomes for Adult Hemophagocytic Lymphohistiocytosis Patients with Different Underlying Diseases

Purpose

Hemophagocytic lymphohistiocytosis (HLH) is a severe disease with high mortality. The purpose of this investigation was to build models to predict 30-day death in total and subgroup HLH patients based on available and cheap laboratory parameters.

Method

The research contained 431 adults HLH patients from January 2015 to September 2021 in the hospital. Logistic regression and receiver operating characteristic (ROC) were utilized to build models.

Results

Results suggested that age, ferritin, lymphocyte (LY), international normalized ratio (INR), thrombin time (TT), globulin, uric acid (UA), chloride, activated partial thromboplastin time (APTT), aspartate aminotransferase (AST), triglycerides (TG), total bilirubin (TB), and indirect bilirubin (IB) were independent factors in HLH and subgroups. Then, models adapted to patients with different underlying diseases were established based on these factors. Area under curve (AUC) of these models was excellent: HLH patients: 0.838 (p < 0.001); infection-associated HLH (I-HLH) patients: 0.913 (p < 0.001); malignancy-associated HLH (M-HLH): 0.921 (p < 0.001) and 0.809 (p < 0.001) for two or more different etiologies-associated HLH (Mix-HLH patients). In addition, UA, TT, and chloride were firstly confirmed as independent factors in adult HLH.

Conclusion

Four models depending on biomarkers that available and affordable in clinical practice were built. With these models, high-risk patients with different underlying diseases could be easily identified.

     

骨折端微动应力对局部碱性成纤维细胞生长因子的影响

背景:研究表明骨折端一定形式的微动可以促进骨痂的形成,但微动影响骨折愈合的分子生物学机制还不明确。目的:研究骨折端微动时应力对断端碱性成纤维细胞生长因子的影响。设计:随机对照的动物实验。单位:解放军第二炮兵总医院骨科,解放军总医院第一附属医院骨科,解放军总医院第一附属医院药剂科。材料:实验于2003-03/2004-04在解放军总医院第一附属医院动物实验室及解放军军事医学科学院八室完成。选取健康纯种新西兰大耳白兔72只,清洁级,月龄5～6个月,体质量2.5～3.0kg,由军事医学科学院动物中心提供。按随机数字表法分为两组:微动组和固定组,每组36只。两组动物又分别分为术后7,14,21,28,42,56d6个时间点进行观察,每个时间点6只。方法:应用氯胺酮、速眠新肌注麻醉所有动物,于胫骨平台下3,3.5,5.5,6cm分别旋入固定针,安装外固定架,夹具距内侧骨皮质1.5cm,胫骨平台下4.5cm处横行截断胫骨,固定组、微动组骨折间隙分别为2.0,2.5mm。固定组动物应用单臂外固定架固定,解剖复位骨折端。微动组动物截骨固定后使外固定架中间杆有0.5mm的轴向移动。术后动物自由行走,依靠自身体质量使外固定架产生微动。术后7,14,21,28,42,56d处死动物。以骨折端为中心,切取1cm长标本,分割,固定12h。术后7,14,21,28,42,56d采用免疫组织化学染色和JVC图像分析处理系统行碱性成纤维细胞生长因子定量分析和显色强度判定。主要观察指标:①碱性成纤维细胞生长因子显色强度判定;②碱性成纤维细胞生长因子定量分析。结果:纳入72只白兔均进入结果分析。碱性成纤维细胞生长因子存在于间质细胞、血管内皮细胞、成骨细胞、成软骨细胞、骨细胞胞浆内表达。术后14,21,28d微动组碱性成纤维细胞生长因子蛋白阳性指数分别为1.98±0.14,2.04±0.12,2.13±0.17,明显大于固定组(1.59±0.14,1.68±0.15,1.63±0.27,P<0.05)。结论:微动应力可使骨折端碱性成纤维细胞生长因子增多,促进骨折愈合。